Open Access Research article

Anatomy and evolution of telomeric and subtelomeric regions in the human protozoan parasite Trypanosoma cruzi

Roberto R Moraes Barros1, Marjorie M Marini1, Cristiane Regina Antônio1, Danielle R Cortez1, Andrea M Miyake1, Fábio M Lima1, Jeronimo C Ruiz2, Daniella C Bartholomeu3, Miguel A Chiurillo4, José Luis Ramirez5 and José Franco da Silveira1*

Author Affiliations

1 Departamento de Microbiologia, Imunologia e Parasitologia Escola Paulista de Medicina, UNIFESP, São Paulo, SP, Brazil

2 Centro de Pesquisas René Rachou, FIOCRUZ-MG, Belo Horizonte, MG, Brazil

3 Departamento de Parasitologia, ICB, UFMG, Belo Horizonte, MG, Brazil

4 Decanato de Ciencias de la Salud, Universidad Centroccidental Lisandro Alvarado (UCLA), Barquisimeto, Venezuela

5 Fundación Instituto de Estudios Avanzados – IDEA, Caracas, Venezuela

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BMC Genomics 2012, 13:229  doi:10.1186/1471-2164-13-229

Published: 8 June 2012

Additional files

Additional file 1 :

Gene annotation ofT. cruzisubtelomeric assemblies. A complete list of chromosome ends, including gene annotation, locus ID, locus size, transcription sense in the chromosome-sized scaffolds (TcChr) and genomic location.

Format: XLS Size: 205KB Download file

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Additional file 2 :

Schematic representation ofT. cruzichromosome ends. Schematic maps of T. cruzi chromosome ends analyzed in this study. The red boxes represent the telomeric repeats (TTAGGG). Each colored box represents a single annotated gene (TriTrypDB - http://tritrypdb.org/tritrypdb/) as indicated in the figure. The maps are to scale and the genomic coordinates are indicated. Blue arrows indicate the transcription sense. Each chromosome end is oriented 5’ to 3’ according to the TriTrypDB annotation. The chromosome ends were separated into eleven distinct groups according to the gene content of the subtelomeric region.

Format: PPTX Size: 612KB Download file

Open Data

Additional file 3 :

Synteny analysis between homologous chromosome ends ofT. cruzi. Synteny analysis between the homologous chromosome ends listed in Table 3. The red lines represent regions of homology between the contigs. The annotated genes are indicated by colored boxes.

Format: PPTX Size: 417KB Download file

Open Data

Additional file 4 :

Subtelomeric DGF-1 genes. A list of DGF-1 genes found in the subtelomeric regions, including annotation (TriTryp DB), locus_id, locus size, chromosome-sized scaffolds (TcChr) location, genomic location and gene integrity.

Format: XLS Size: 38KB Download file

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Additional file 5 :

Subtelomeric RHS genes. A complete list of RHS family genes found in the subtelomeric regions, including annotation (TriTrypDB), locus_id, locus size, chromosome-sized scaffolds (TcChr) location, genomic location and gene integrity.

Format: XLS Size: 48KB Download file

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Additional file 6 :

Subtelomeric retrotransposons. A complete list of retroelements identified in the subtelomeric regions, including annotation (GIRI DB), locus size, chromosome-sized scaffolds (TcChr) location, genomic location and gene integrity.

Format: XLS Size: 33KB Download file

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Additional file 7 :

Subtelomeric trans-sialidases (TS). A complete list of trans-sialidase family members identified in the subtelomeric regions, including locus_id, locus size, chromosome-sized scaffolds (TcChr) location, genomic location, transcript classification, gene integrity and classification according Freitas et al. [23].

Format: XLS Size: 49KB Download file

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Additional file 8 :

Evidence for translation of subtelomeric genes. A complete list of genes identified by mass spectrometry-based evidence of gene expression of TriTrypDB, including locus_id, locus size, chromosome-sized scaffolds (TcChr) location, genomic location, gene annotation and life cycle stage in which the peptide was isolated.

Format: XLS Size: 34KB Download file

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Open Data