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Open Access Research article

Genomic characterization of two Staphylococcus epidermidis bacteriophages with anti-biofilm potential

Diana Gutiérrez, Beatriz Martínez, Ana Rodríguez and Pilar García*

Author Affiliations

DairySafe Group, Department of Technology and Biotechnology of Dairy Products, Instituto de Productos Lácteos de Asturias (IPLA-CSIC), Paseo Río Linares, s/n, 33300, Villaviciosa, Asturias, Spain

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BMC Genomics 2012, 13:228  doi:10.1186/1471-2164-13-228

Published: 8 June 2012

Additional files

Additional file 1:

Table S1. Features of bacteriophage phi-IPLA5 orfs, gene products (gp) and functional assignments.

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Additional file 2:

Table S2. Features of bacteriophage phi-IPLA7 orfs, gene products (gp) and functional assignments.

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Additional file 3:

Table S3. Putative promoters and terminators sequences of phi-IPLA5 and phi-IPLA7. -10 and -35 boxes are underlined. Nucleotide positions and presence of the TG dinucleotide were also indicated. At terminator sequences nucleotides in the stem-loop structure are underlined.

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Additional file 4:

Table S4. Primers used for multiplex PCR reactions.

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Additional file 5:

Figure S1. Multiplex PCR detecting the integrase, holin and major head protein genes in S. epidermidis strains. (H) Strains isolated from healthy woman, (M) strains isolated from mastitic women. Absence and presence of a specific gene is represented by white and grey boxes, respectively.

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