Figure 4.

Effects of hyperoxia on the expression of miR-29 and predicted mRNA targets. (A) Relative expression of miR-29a and miR-29c (n = 3, mean ± SE, # p < 0.05 vs. P14 room air controls, * p < 0.05 vs. P29 room air controls). (B) Relative mRNA expression by RT-PCR comparing mouse lung exposed to room air with hyperoxia (n = 3, mean ± SE, # p < 0.05 vs. P14 room air controls, * p < 0.05 vs. P29 room air controls). (C) Nrtk2 protein expression measured by Western blot analysis using β-actin as the loading control (n = 3, mean ± SE, # p < 0.05 vs. P14 room air controls, * p < 0.05 vs. P29 room air controls). Protein expression of Nrtk2 in the lung of P14-RA (D), P14-O₂-treated (E), P29-RA (F) and P29-O₂-treated mice (G). Nrtk2 immunoreactivity was detected in brown. (H) and (I) Immunohistochemical localization of Nrtk2 in P29-RA lung. Red arrow indicates bronchiolar epithelial cells, green arrow indicates type II pneumocyte, blue indicates endothelial cells and black indicates macrophage.