Open Access Research article

MicroRNA-mRNA interactions in a murine model of hyperoxia-induced bronchopulmonary dysplasia

Jie Dong15, William A Carey2, Stuart Abel15, Christopher Collura25, Guoqian Jiang4, Sandra Tomaszek15, Shari Sutor15, Anja C Roden6, Yan W Asmann4, Y S Prakash35 and Dennis A Wigle15*

Author Affiliations

1 Division of General Thoracic Surgery, Department of Surgery, Mayo Clinic, Rochester, MN, USA

2 Division of Neonatal Medicine, Department of Pediatrics, Mayo Clinic, Rochester, MN, USA

3 Department of Anesthesiology, Mayo Clinic, Rochester, MN, USA

4 Division of Biomedical Statistics and Informatics, Department of Health Sciences Research, College of Medicine, Mayo Clinic, Rochester, MN, USA

5 Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, USA

6 Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA

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BMC Genomics 2012, 13:204  doi:10.1186/1471-2164-13-204

Published: 30 May 2012

Additional files

Additional file 1::

Figure S1. Relative miR-29 expression in BASC cells after transfection of miR-29 mimics.

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Additional file 2::

Table S5. MiR-29 targeted gene expression in BASC cells following transfection with miR-29 mimics.

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Additional file 3::

Table S1. Identification of functionally related clusters in hyperoxia-induced BPD. All 2714 mRNA probes which significantly changed under hyperoxia were classified into functionally related clusters using DAVID. The 6 top functionally related clusters including cell cycle, adhesion, mobility, angiogenesis, taxis, and inflammation are listed.

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Additional file 4::

Table S2. List of hyperoxia-responsive genes involved in functional categories of cell cycle, adhesion, mobility, migration, angiogenesis, taxis and inflammation. Potential miRNAs involved in regulation of these functions are also listed.

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Additional file 5::

Table S3. Overlap of dynamically regulated miRNAs in normal lung alveolar development and hyperoxia-induced BPD. Among 66 miRNAs identified from O2-induced BPD, 33 miRNAs overlapped with the 117 miRNA identified as dynamically regulated during lung organogenesis. The expressed copy number values of all 117 miRNA are listed.

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Additional file 6::

Table S4. Primer sequences for real-time PCR.

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