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Open Access Research article

MicroRNA-mRNA interactions in a murine model of hyperoxia-induced bronchopulmonary dysplasia

Jie Dong15, William A Carey2, Stuart Abel15, Christopher Collura25, Guoqian Jiang4, Sandra Tomaszek15, Shari Sutor15, Anja C Roden6, Yan W Asmann4, Y S Prakash35 and Dennis A Wigle15*

Author affiliations

1 Division of General Thoracic Surgery, Department of Surgery, Mayo Clinic, Rochester, MN, USA

2 Division of Neonatal Medicine, Department of Pediatrics, Mayo Clinic, Rochester, MN, USA

3 Department of Anesthesiology, Mayo Clinic, Rochester, MN, USA

4 Division of Biomedical Statistics and Informatics, Department of Health Sciences Research, College of Medicine, Mayo Clinic, Rochester, MN, USA

5 Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, USA

6 Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA

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Citation and License

BMC Genomics 2012, 13:204  doi:10.1186/1471-2164-13-204

Published: 30 May 2012

Abstract

Background

Bronchopulmonary dysplasia is a chronic lung disease of premature neonates characterized by arrested pulmonary alveolar development. There is increasing evidence that microRNAs (miRNAs) regulate translation of messenger RNAs (mRNAs) during lung organogenesis. The potential role of miRNAs in the pathogenesis of BPD is unclear.

Results

Following exposure of neonatal mice to 80% O2 or room air (RA) for either 14 or 29 days, lungs of hyperoxic mice displayed histological changes consistent with BPD. Comprehensive miRNA and mRNA profiling was performed using lung tissue from both O2 and RA treated mice, identifying a number of dynamically regulated miRNAs and associated mRNA target genes. Gene ontology enrichment and pathway analysis revealed that hyperoxia modulated genes involved in a variety of lung developmental processes, including cell cycle, cell adhesion, mobility and taxis, inflammation, and angiogenesis. MiR-29 was prominently increased in the lungs of hyperoxic mice, and several predicted mRNA targets of miR-29 were validated with real-time PCR, western blotting and immunohistochemistry. Direct miR-29 targets were further validated in vitro using bronchoalveolar stem cells.

Conclusion

In newborn mice, prolonged hyperoxia induces an arrest of alveolar development similar to that seen in human neonates with BPD. This abnormal lung development is accompanied by significant increases in the levels of multiple miRNAs and corresponding decreases in the levels of predicted mRNA targets, many of which have known or suspected roles in pathways altered in BPD. These data support the hypothesis that dynamic regulation of miRNAs plays a prominent role in the pathophysiology of BPD.