Additional file 14.

Detection of the pSZ77repAgene andGeobacteraceaespp. 16S rRNA genes in pure and mixed cultures containing Geobacter strains with specific PCR primers. Top row: repA gene-targeted PCR (565 bp amplicon) and bottom row Geobacteraceae 16S rRNA gene-targeted PCR (312 bp amplicon). The arrows indicate the expected PCR amplicons. Lane 1: 1 kb Plus DNA ladder (Invitrogen), Lane 2: no template (negative control), Lane 3: G. lovleyi strain SZ (positive control), Lanes 4 and 5: consortium BDI, Lane 6: consortium KB-1, Lane 7: Ft. Lewis isolate 7.1, Lane 8: Ft. Lewis isolate 7.2, Lane 9: Ft. Lewis isolate 7.3, Lane 10: Ft. Lewis isolate 7.4, Lane 11: G. thiogenes.

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Wagner et al. BMC Genomics 2012 13:200   doi:10.1186/1471-2164-13-200