Predicted replicon region of pSZ77. The DnaA-binding sites found at a low-GC region 3' to the predicted repA ORF provide evidence that this region is the plasmid origin of replication (oriR). The repeats are not closely spaced, and do not resemble the tandem repeats found in the plasmids of Geobacter metallireducens and Pelobacter propionicus. Protein coding genes (arrows) are as follows: repA‒predicted replication initiation protein (yellow), parA‒predicted partitioning ATPase (green), cobD/cbiP: cobalamin synthesis pathway gene (red). The two unshaded genes are annotated as histone-binding proteins. Non-coding regions of interest (rectangles) are as follows: black = predicted DnaA protein-binding site, TT(C|A)TCCAC(A|G), grey = repeat 1, CGTGACAATTCATGC, white = repeat 2, TGTATAGACAATACAATA.
Wagner et al. BMC Genomics 2012 13:200 doi:10.1186/1471-2164-13-200