Expression analysis of Gli-target candidate genes in control and cyc treated-cells. Real-time RT-PCR analysis of expression of genes control gene ptc1 (Patched1), cdh13 (cadherin 13), neo1 (Neogenin 1), sfrp2 (Secreted Frizzled Related Protein 2), rps6ka3 (ribosomal protein S6 kinase polypeptide 3), rps6ka1 (ribosomal protein S6 kinase polypeptide 1), c-myc (myelocytomatosis oncogene), kif2 (Kinesin Heavy Chain Member 2), itga1 (integrin alpha 1) and glra2 (glycine receptor, alpha 2 subunit) in control cells and in cells treated with 10 μM cyc or control (vehicle EtOH) for 6, 12 or 24 hours before harvesting. Data shown are transcript levels relative to housekeeping gene gapdh (glyceraldehyde 3-phospate dehydrogenase). Values correspond to the mean and SE of at least triplicate determinations and asterisks indicate significant difference between control and treated cells (ANOVA, p < 0.05). Black bars, control samples and white bars, inhibitor treated samples.
Milla et al. BMC Genomics 2012 13:2 doi:10.1186/1471-2164-13-2