Additional file 2.

Table S1. The Elution Conditions of HPLC Analysis. Note: T, Retention time; A, mobile phase deionized water- formic acid (99:1, v/v); B, mobile phase methanol. Table S2. Formula of Active Compound Content. Note: Calibration plots of eight standards were constructed on the basis of peak areas (y) using seven different concentration solutions (x). All plots were linear in the examined ranges, and linear ranges had been shown as the concentration of the standard compounds (μg mL-1). The r referred to the correlation coefficient of the equation. The standard compounds Chlorogenic acid (110753), Caffeic acid(110885), ferulic acid(110773), Rutin(100080), Luteoloside(111720), Hyperoside(111521), Quercitrin(111538) and Quercetin (100081) were purchased from National institutes for food and drug control, China. Table S3. Number of Contigs in KEGG Pathways. Note: FLJ, Lonicera japonica Thumb; rFLJ, Lonicera japonica Thunb. var. chinensis (Wats.); B, bud; F1,flower1; F2, flower2. Table S4. Predicted Phosphorylated Sites in 34 Protein Sequence from Differential Express Gene. Note: Predicted Phosphorylated Sites using software online (http://kinasephos2.mbc.nctu.edu.tw/) and protein sequence was perdicted by ORF finder (http://www.ncbi.nlm.nih.gov/gorf/gorf.html webcite). Table S5. qRT-PCR and RNA-seq Analysis of Gene Express between Buds ofLonicera japonicaThunb. var. chinensis (Wats.) andLonicera japonicaThumb. Note: RR/YR, the ratio of transcripted level in buds of Lonicera japonica Thunb. var. chinensis (Wats.) and Lonicera japonica Thumb. Table S6. Orthologs Identity of Differential Express Genes Sequence between FLJ and rFLJ. Note: FLJ, Lonicera japonica Thumb; rFLJ, Lonicera japonica Thunb. var. chinensis (Wats.) Table S7 PAL, CHS, HMGR and PK Gene Families in Arabidopsis and Grape. Table S8. Putative Enzyme Pool to Control the Active Compounds in Buds ofLonicera japonicaThumb.

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Yuan et al. BMC Genomics 2012 13:195   doi:10.1186/1471-2164-13-195