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Open Access Highly Accessed Research article

Genetic variation and metabolic pathway intricacy govern the active compound content and quality of the Chinese medicinal plant Lonicera japonica thunb

Yuan Yuan1, Lipu Song2, Minhui Li14, Guiming Liu2, Yanan Chu2, Luyu Ma3, Yuanyuan Zhou2, Xiao Wang15, Wei Gao1, Shuangshuang Qin1, Jun Yu2, Xumin Wang2* and Luqi Huang1*

Author Affiliations

1 Beijing Key Laboratory of Functional Genomics for Dao-di Herbs,Institute of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing, 100700, China

2 CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100029, China

3 Shandong Academy of Medical Sciences, Jinan, 250031, China

4 Baotou Medical College, Baotou, 014040, China

5 Shandong Analysis and Test center, Jinan, 250014, China

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BMC Genomics 2012, 13:195  doi:10.1186/1471-2164-13-195

Published: 20 May 2012

Abstract

Background

Traditional Chinese medicine uses various herbs for the treatment of various diseases for thousands of years and it is now time to assess the characteristics and effectiveness of these medicinal plants based on modern genetic and molecular tools. The herb Flos Lonicerae Japonicae (FLJ or Lonicera japonica Thunb.) is used as an anti-inflammatory agent but the chemical quality of FLJ and its medicinal efficacy has not been consistent. Here, we analyzed the transcriptomes and metabolic pathways to evaluate the active medicinal compounds in FLJ and hope that this approach can be used for a variety of medicinal herbs in the future.

Results

We assess transcriptomic differences between FLJ and L. japonica Thunb. var. chinensis (Watts) (rFLJ), which may explain the variable medicinal effects. We acquired transcriptomic data (over 100 million reads) from the two herbs, using RNA-seq method and the Illumina GAII platform. The transcriptomic profiles contain over 6,000 expressed sequence tags (ESTs) for each of the three flower development stages from FLJ, as well as comparable amount of ESTs from the rFLJ flower bud. To elucidate enzymatic divergence on biosynthetic pathways between the two varieties, we correlated genes and their expression profiles to known metabolic activities involving the relevant active compounds, including phenolic acids, flavonoids, terpenoids, and fatty acids. We also analyzed the diversification of genes that process the active compounds to distinguish orthologs and paralogs together with the pathways concerning biosynthesis of phenolic acid and its connections with other related pathways.

Conclusions

Our study provides both an initial description of gene expression profiles in flowers of FLJ and its counterfeit rFLJ and the enzyme pool that can be used to evaluate FLJ quality. Detailed molecular-level analyses allow us to decipher the relationship between metabolic pathways involved in processing active medicinal compounds and gene expressions of their processing enzymes. Our evolutionary analysis revealed specific functional divergence of orthologs and paralogs, which lead to variation in gene functions that govern the profile of active compounds.

Keywords:
RNA-seq; Transcriptome; Active compounds; Synthetic pathways; Flos Lonicerae Japonicae