Open Access Research article

Transcription profiling reveals stage- and function-dependent expression patterns in the filarial nematode Brugia malayi

Ben-Wen Li14*, Zhengyuan Wang2, Amy C Rush1, Makedonka Mitreva23 and Gary J Weil1

Author Affiliations

1 Infectious Diseases Division, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO, 63110, USA

2 The Genome Institute, Washington University School of Medicine, St. Louis, MO, 63110, USA

3 Department of Genetics, Washington University School of Medicine, St. Louis, MO, 63110, USA

4 Washington University School of Medicine, Campus Box 8051, 660 S. Euclid, St. Louis, MO, 63110, USA

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BMC Genomics 2012, 13:184  doi:10.1186/1471-2164-13-184

Published: 14 May 2012

Additional files

Additional file 1:

The schema of B. malayi lifecycle and stages studied in current study. Figure S1. This figure shows the life cycle stages of Brugia malayi and lifecycle stages included in current study. The life cycle of the filarial parasite is digenetic with a mammalian host (light green) and a mosquito intermediate host (green). Infections are initiated after a mosquito blood meal when the infective L3 stage enter the mammalian host. L3 larvae migrate to lymphatic vessels, develop, and molt twice to transform into adult parasites. The adult parasites mate, produce microfilariae (Mf) that are released into the circulation and ingested by mosquitoes during a blood meal. The ingested microfilariae molt twice and develop into the infective L3 stage in mosquito vector. Note: Stages analyzed in this study are marked in black circles.

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Additional file 2:

Transcripts expressed in any stage and gender.

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Additional file 3:

Differentially expressed transcripts (DE) identified across the stages studied (adj P  < 0.05).

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Additional file 4:

Five transcriptional patterns identified by K-means clustering.

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Additional file 5:

Potential filarial homologues of C. elegans genes annotated as “spermatogenesis- and oogenesis-enriched” identified in the clusters.

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Additional file 6:

Transcriptional profiles of gene family identified in global transcription data.

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Additional file 7:

Differentially expressed transcripts identified in pairwise comparisons.

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Additional file 8:

Potential filarial homologues of C. elegans genes annotated as “dauer-regulated and dauer-enriched” identified in L3 upregulated transcripts vs. Mf.

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Additional file 9:

Potential filarial homologues of C. elegans genes annotated as spermatogenesis-, oogenesis-, embryogenesis- and germline-enriched identified within data sets: upregulated transcripts in 6WM vs. L4, upregulated transcripts in 6WF vs. L4, upregulated transcripts in AM vs. 6WM and upregulated transcripts in AF vs. 6WF.

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Additional file 10:

GO associations of transcripts constitutively expressed across the stages studied.

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Additional file 11:

GO associations of transcripts expressed in five gene clusters.

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Additional file 12:

GO associations of transcripts differentially expressed in pairwise comparisons.

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Additional file 13:

Expressed transcripts with encoded proteins identified by previous proteomic studies.

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