Proteomic tree of ten genus Lactobacillus species. There are ten Lactobacillus species known to have fermentative abilities according to Bergey's Manual of Systematic Bacteriology . Some of them obtain energy through homo fermentation while others through hetero fermentation. Since these species are very closely-related, traditionally, biochemical analyses would be performed to distinguish or classify them. By using the proposed probe-set method, these species are clustered into two groups. As indicated by the dotted line, these two groups respectively consist of homo and hetero fermentative species. This result demonstrates the feasibility of the probe-set method as a metabolic classifier, which is especially useful for classifying closely-related species that are difficult to distinguish by biomarkers.
Lee et al. BMC Genomics 2012 13:157 doi:10.1186/1471-2164-13-157