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Open Access Highly Accessed Research article

Characterization of the transcriptome profiles related to globin gene switching during in vitro erythroid maturation

Biaoru Li, Lianghao Ding, Wei Li, Michael D Story and Betty S Pace*

BMC Genomics 2012, 13:153  doi:10.1186/1471-2164-13-153

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Can it really achieve globin switching in vitro within one-phase system?

Wentian Wang   (2013-03-10 13:10)  pumc email

see below

Competing interests

First, I can not blieve erythropoiesis under 34-day induction with high EPO and other cell factors. In my experience, erythropoiesis will end in 2-3 weeks.it is too long to sustain differenetiation. Second, in one-phase system, it can not realize globin switching spontaneously, at least I supposed so. There is a incompatible result from another reference this year, in which the globin switching can only happen under feeder cell co-culture system. http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0036846 So, which one is the right veritas?

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