Semi-quantitative RT-PCR analysis of 12 selected up-regulated unigenes. Semi-quantitative RT-PCR was carried out using RNA from the Taxus cuspidata P93AF cell line. RNA samples were taken at 6h, 18h and 5 day after MJ elicitation, and from mock elicited cells at the same time points. Lane 1: Mock elicited for 18h, Lane 2-4: elicited with 100 μM MJ for 6h, 18h or 5 day, respectively. Gapdh and Actin1 were used as normalization controls.
Lenka et al. BMC Genomics 2012 13:148 doi:10.1186/1471-2164-13-148