Additional file 1.

(A). Pax8 chromatin immunoprecipitation validation performed to confirm enrichment of target DNA fragments by means of real-time PCR. Sequences belonging to the Nis upstream enhancer element (NUE) [7] and Tpo promoter sequences [9], previously described as Pax8 binding sites in rat thyroid cells, were used as positive controls. (B). ChIP-Seq results with regard to the Nis locus were visualized in the UCSC genome browser. Significant immunoprecipitated peak corresponding to MACS program included the Nis upstream enhancer (NUE), previously described to be regulated by Pax8 (underlined red letters).

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Ruiz-Llorente et al. BMC Genomics 2012 13:147   doi:10.1186/1471-2164-13-147