Additional file 1.
Figure S1. SNP validation by high resolution melting (HRM) curve analysis (A) and direct sequencing of PCR fragments (B). (A) shows the normalized melting curves of a target SNP for twelve mapping individuals along with the parental genotypes that were used for short amplicon melting as described by Studer et al. . The melting curves given in grey represent individuals being homozygous for the target SNP, while red melting curves indicated heterozygous individuals. The sequencing trace file given in (B) illustrates the results from direct sequencing of PCR products amplified from the parental genotype being heterozygous for the target SNP. Sequencing of PCR fragments was performed at Eurofins MWG Operon, Ebersberg, Germany.
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Studer et al. BMC Genomics 2012 13:140 doi:10.1186/1471-2164-13-140