Table 3

qPCR validation of results and microarray data obtained from 29-50 hr larvae

microarray

qPCR

microarray

qPCR


ManfΔ96vs w

ManfΔ96vs w

ManfΔ96 vs 69B>Manf133

69B>Manf133 vs w

69B>Manf133 vs w


#

Gene Name

log FC

P.Value

log2

T-test

log FC

P.Value

log FC

P,Value

log2

T-test

gene ID


1

DmManf

-8.0

0.0001

-2.0

0.0002

3.2

6.0E-07

CG7013


2

CG14879

-3.2

5.5E-06

-11.5

0.0002

-2.7

4.4E-05

-3.9

1.5E-06

CG14879


3

cycle

-0.5

0.0002

-7.2

0.0001

-1.0

4.7E-09

0.5

3.2E-05

-5.0

9.0E-08

CG8727


4

nol

-1.1

0.0003

-4.3

1.7E-05

-1.9

3.9E-07

1.1

6.3E-06

0.8

0.0005

CG32077


5

Pak3

-2.8

3.1E-07

-5.8

5.2E-05

-2.7

3.8E-07

CG14895


6

pipe

-0.9

0.0003

-1.2

3.7E-06

-1.0

1.8E-05

0.9

4.1E-05

CG9614


7

Rala

-1.4

2.4E-08

-2.0

0.0001

1.4

5.7E-09

0.9

4.2E-06

CG2849


8

Rep

-2.0

0.0001

-3.3

4.6E-07

CG8432


9

sip3

-0.5

0.0003

-9.6

6.4E-06

CG1937


10

Sk2

-0.6

0.0007

-8.7

1.2E-05

-1.6

0.0003

0.6

0.0003

-12.2

4.5E-08

CG32484


11

Ubc-E2H

0.7

0.0001

1.2

9.9E-05

CG2257


12

Pi3K

-1.0

5.5E-05

-9.9

0.0003

-1.0

8.0E-05

TC208938


13

Uch-L3

-0.6

0.0003

-0.4

0.0001

-0.4

0.0004

CG3431


Only statistically significant (P < 0.01) results are presented. Results of qPCR results were made comparable to microarray fold change (FC) by calculating the log2 of the relative fold changes. T-test means calculated P-value associated with Student's T-test.

Palgi et al. BMC Genomics 2012 13:134   doi:10.1186/1471-2164-13-134

Open Data