Open Access Highly Accessed Research article

De novo assembly and characterization of the root transcriptome of Aegilops variabilis during an interaction with the cereal cyst nematode

De-Lin Xu12, Hai Long1*, Jun-Jun Liang12, Jie Zhang12, Xin Chen12, Jing-Liang Li12, Zhi-Fen Pan1, Guang-Bing Deng1 and Mao-Qun Yu1*

Author Affiliations

1 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan, China

2 Graduate University of the Chinese Academy of Sciences, Beijing, China

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BMC Genomics 2012, 13:133  doi:10.1186/1471-2164-13-133

Published: 11 April 2012

Additional files

Additional file 1:

Roots invaded by CCN. A prep-experiment confirmed the CCN J2 could parasitize plant root effectively before RNA extraction. Few hours later after the CCN inoculation, one nematode was detected being piercing root epidermis (Figure S1a). Utilizing the microscope, one CCN J2 was found invading into a root tip of plant, already (Figure S1b; part of the CCN cover was removed).

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Additional file 2:

Resistance related unigenes from GO classification CCN.

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Additional file 3:

KEGG pathway mapping.

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Additional file 4:

Defending unigenes from COG alignment.

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Additional file 5:

Unigene annotations in public databases.

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Additional file 6:

Nematode-like unigenes list in the transcriptome database.

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Additional file 7:

Resistance candidate unigenes in this study.

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Additional file 8:

Unigenes involved in plant-pathogen interaction pathway.

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Additional file 9:

Unigenes involved in phosphatidy linositol signaling system pathway.

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Additional file 10:

Unigenes involved in ABC transporters pathway.

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