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Open Access Highly Accessed Research article

Transcriptome-wide identification and characterization of miRNAs from Pinus densata

Li-Chuan Wan1, Haiyan Zhang1, Shanfa Lu2, Liang Zhang13, Zongbo Qiu1, Yuanyuan Zhao13, Qing-Yin Zeng4* and Jinxing Lin1*

Author Affiliations

1 Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

2 Medicinal Plant Cultivation Research Center, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, Haidian District 100193, China

3 Graduate School of the Chinese Academy of Sciences, Beijing 100049, China

4 State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

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BMC Genomics 2012, 13:132  doi:10.1186/1471-2164-13-132

Published: 6 April 2012

Additional files

Additional file 1:

Precursor sequences of P. densata conserved miRNAs.

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Open Data

Additional file 2:

Primary sequences of P. densata conserved miRNAs.

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Additional file 3:

Predicted hairpin structures of P. densata miRNA precursors. The hairpin structures of P. densata miRNA precursors were predicted by MFOLD. Mature miRNAs were marked in yellow.

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Open Data

Additional file 4:

Predicted stem-loop structures of precursors containing P. densata microRNA sequences (red and blue).

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Additional file 5:

Conserved mature miRNAs without hairpin structure in P. densata.

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Additional file 6:

Predicted targets of P. densata miRNAs and their putative functions.

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Additional file 7:

Primers used for amplifying P. densata miRNAs and their targets.

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