Open Access Research article

Variation in genomic islands contribute to genome plasticity in Cupriavidus metallidurans

Rob Van Houdt1*, Pieter Monsieurs1, Kristel Mijnendonckx12, Ann Provoost1, Ann Janssen1, Max Mergeay1 and Natalie Leys1

Author Affiliations

1 Unit of Microbiology, Belgian Nuclear Research Centre (SCK•CEN), B-2400 Mol, Belgium

2 Laboratory of Food and Environmental Microbiology, Université catholique de Louvain, B-1348 Louvain-la-Neuve, Belgium

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BMC Genomics 2012, 13:111  doi:10.1186/1471-2164-13-111

Published: 23 March 2012

Additional files

Additional file 1:

Figure S1. Plasmid patterns of C. metallidurans strains. Agarose gel electrophoresis of plasmid extracts from strains CH34 (1 and 18), KT01 (2), KT02 (3), KT21 (4), CH42 (5), CH79 (6), AS39 (7), AS167 (8), AS168 (9), 31A (10), SV661 (11), 43015 (12), 45957 (13), NE12 (14), NA1 (15), NA2 (16), and NA4 (17). Lower band represents chromosomal DNA (PDF 33 kb).

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Additional file 2:

Word document containing the Supplementary Tables 1 to 5 (DOC 146 kb).

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Additional file 3:

Figure S2. Cartographic map of chromosome 2 of the different C. metallidurans strains. Negative hybridization signals are highlighted red. Newly identified putative genomic islands are indicated by blue bars (PDF 23 kb).

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Additional file 4:

Figure S3. Cartographic map of chromosome 1 of the different C. metallidurans strains. Negative hybridization signals are highlighted red. Previously identified genomic islands are indicated by dark blue bars. Newly identified putative genomic islands are indicated by green bars (PDF 33 kb).

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Additional file 5:

Table S6. (excel document) with the results of the comparative genomic hybridization (XLS 2902 kb).

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