ORF filtering vector. The filtering vectors features are shown in panel A. Blunt ended gDNA random fragments are cloned between a leader sequence (Sec, SRP, or TAT) and the mature β-lactamase gene. C-terminal SV5 and His tags are used for detection and purification respectively. In panel B, the effect of selective pressure on ampicillin is shown for ORFs and non ORFs.
D'Angelo et al. BMC Genomics 2011 12(Suppl 1):S5 doi:10.1186/1471-2164-12-S1-S5