This article is part of the supplement: Validation methods for functional genome annotation
Research
Filtering "genic" open reading frames from genomic DNA samples for advanced annotation
- † Equal contributors
1 Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM, USA
2 Department of Structural Chemistry and Inorganic Stereochemistry, School of Pharmacy, University of Milan, Milan, Italy
3 Department of Medical Sciences and IRCAD, University of Eastern Piedmont, Novara, Italy
BMC Genomics 2011, 12(Suppl 1):S5 doi:10.1186/1471-2164-12-S1-S5
Published: 15 June 2011Additional files
Additional file 1:
β-lactamase assay on TAT library. Panel A shows 45-48 TAT clones for each filtering concentration in the 96 well format nitrocefin (NC) assay. Clones correctly expressing a functional β-lactamase fusion in the supernatant turn from yellow to red. Controls are shown in black circles. In panel B, absorbance measurements performed at 2 h, 6 h, and 16 h (saturation point) are shown as average measurement of triplicate plates. Data were normalized on the positive control signal (shown as black bar with 100% signal) and reported as percentage value.
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