This article is part of the supplement: Validation methods for functional genome annotation

Open Access Research

Filtering "genic" open reading frames from genomic DNA samples for advanced annotation

Sara D'Angelo1, Nileena Velappan1, Flavio Mignone2, Claudio Santoro3, Daniele Sblattero3, Csaba Kiss1 and Andrew RM Bradbury1*

  • * Corresponding author: Andrew RM Bradbury amb@lanl.gov

  • † Equal contributors

Author Affiliations

1 Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM, USA

2 Department of Structural Chemistry and Inorganic Stereochemistry, School of Pharmacy, University of Milan, Milan, Italy

3 Department of Medical Sciences and IRCAD, University of Eastern Piedmont, Novara, Italy

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BMC Genomics 2011, 12(Suppl 1):S5  doi:10.1186/1471-2164-12-S1-S5

Published: 15 June 2011

Additional files

Additional file 1:

β-lactamase assay on TAT library. Panel A shows 45-48 TAT clones for each filtering concentration in the 96 well format nitrocefin (NC) assay. Clones correctly expressing a functional β-lactamase fusion in the supernatant turn from yellow to red. Controls are shown in black circles. In panel B, absorbance measurements performed at 2 h, 6 h, and 16 h (saturation point) are shown as average measurement of triplicate plates. Data were normalized on the positive control signal (shown as black bar with 100% signal) and reported as percentage value.

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