Functional annotation of C. reinhardtii JGI v4.0 translated ORFs. Enzymatic functions were assigned to the JGI v4.0 translated ORFs by comparing the sequences with the UniProt and AraCyc enzyme databases. The computational pipeline (A) entailed transfer of enzyme annotation to JGI ORFs identified through reciprocal BLAST, then establishing paralog groups to extend enzyme annotation to paralogs. Our functional annotation identified 886 EC numbers, of which only ~50% are currently annotated by KEGG (B).
Ghamsari et al. BMC Genomics 2011 12(Suppl 1):S4 doi:10.1186/1471-2164-12-S1-S4