Open Access Research article

An Expressed Sequence Tag collection from the male antennae of the Noctuid moth Spodoptera littoralis: a resource for olfactory and pheromone detection research

Fabrice Legeai1, Sébastien Malpel25, Nicolas Montagné2, Christelle Monsempes2, François Cousserans3, Christine Merlin26, Marie-Christine François2, Martine Maïbèche-Coisné2, Frédérick Gavory4, Julie Poulain4 and Emmanuelle Jacquin-Joly2*

Author Affiliations

1 IRISA, équipe Symbiose, Campus universitaire de Beaulieu, 35042 Rennes Cedex, France

2 INRA, UMR-A 1272 INRA-UPMC PISC Physiologie de l'Insecte: Signalisation et Communication, route de Saint-Cyr, 78026 Versailles Cedex, France

3 INRA, UMR 1231 INRA-Université Montpellier II BIVI, Place Eugène Bataillon, 34095 Montpellier Cedex 5, France

4 Genoscope, Centre National de Séquençage, 2 rue Gaston Crémieux, BP 191, F-91057 Evry Cedex, France

5 UMR 5548, University of Bourgogne, 6 bd Gabriel, F-21000 Dijon, France

6 Department of Neurobiology, University of Massachusetts Medical School, 01604, Worcester, MA, USA

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BMC Genomics 2011, 12:86  doi:10.1186/1471-2164-12-86

Published: 29 January 2011

Additional files

Additional file 1:

Full description of S. littoralis ESTs, including accession number, sequence, length, accession number of the corresponding contig if any and blast hit.

Format: ZIP Size: 4.5MB Download file

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Additional file 2:

Full description of S. littoralis unigenes, including accession number, gene size, ORF length, EST coverage, blast, signalP analysis, unigene sequence, ORF sequence.

Format: XLS Size: 16.3MB Download file

This file can be viewed with: Microsoft Excel Viewer

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Additional file 3:

Table of the GO terms over or under represented in S. littoralis ORFs having no similarity with the B. mori proteome. Test group: ORFs with no match. Reference group: ORFs with at least one match. Fisher's exact test with multiple testing correction.

Format: DOC Size: 261KB Download file

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Additional file 4:

Table of the Interproscan results for the 678 S. littoralis unigene sequences having no similarity with any known proteins.

Format: DOC Size: 144KB Download file

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Additional file 5:

Table of the GO terms over or under represented in S. littoralis unigenes having no lepidopteran EST match. Test group: S. littoralis ESTs with no match. Reference group: S. littoralis ESTs with at least one match. Fisher's exact test with multiple testing correction.

Format: DOC Size: 269KB Download file

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Additional file 6:

Accession numbers for amino acid sequences of the lepidopteran odorant-binding proteins, antennal binding proteins, chemosensory proteins and olfactory receptors used in the phylogenetic analyses.

Format: DOC Size: 118KB Download file

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Additional file 7:

Table reporting the forward and reverse primer sequences used in real-time PCR, annealing temperatures, resulting amplicon lengths and PCR efficiencies.

Format: DOC Size: 64KB Download file

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