Truncation of LkARSs to narrow down essential functional regions. (A-C) Sub-fragments of three LkARSs (two more shown in Additional File 2, Figure S1) were cloned and tested for ARS function. Black boxes represent functional fragments. Red boxes represent non-functional fragments. For each ARS, the position of the best match to the 9 bp LkACS is indicated by a blue box. The extent of the truncation in basepairs is indicated on the left of the graphics (L = truncated from the left, R = truncated from the right). The length of the original full-length fragment isolated from the screen is indicated next to the first fragment from the top. *: This fragment retains very weak ARS activity.
Liachko et al. BMC Genomics 2011 12:633 doi:10.1186/1471-2164-12-633