Table 2

Selected GOa biological process classifications of proteins differentially expressed in HCAECs following cold-adaptation.

# of Proteins

UP

DN

Ratiob

%c

Gene ontology categories

P-Valued

Folde


34

67

-2.0

56.7

Primary metabolic process

1.4 × 10-3

1.1

22

65

-3.0

48.9

Macromolecule metabolic process

1.9 × 10-3

1.3

12

50

-4.2

34.8

Gene expression

3.0 × 10-6

1.7

13

47

-3.6

33.7

Biosynthetic process

1.7 × 10-3

1.4

15

6

2.5

11.8

Catabolic process

9.9 × 10-2

1.4

11

1

11.0

6.7

Glycolysis

5.1 × 10-12

21.6

7

59

-8.4

31.5

Protein metabolic process

3.9 × 10-5

1.7

2

16

-8.0

10.1

Macromolecular complex assembly

2.2 × 10-3

2.3

0

11

- ∞

6.2

Ribonucleoprotein complex assembly

5.3 × 10-5

5.2

9

18

-2.0

15.2

RNA processing

1.1 × 10-9

4.2

8

11

-1.4

10.7

mRNA metabolic process

3.6 × 10-7

4.3

2

38

-19.0

22.5

Translation

2.8 × 10-28

10.2

5

11

-2.2

9.0

Intracellular transport

1.1 × 10-2

2.1

0

7

- ∞

3.9

Protein folding

1.8 × 10-2

3.3

0

9

- ∞

5.1

Unfolded protein bindingf

5.0 × 10-5

6.9

6

5

1.2

6.2

Cell proliferation

3.3 × 10-2

2.1

6

1

6.0

3.9

Anti-apoptosis

3.5 × 10-2

2.9

3

3

1.0

3.4

Structure-specific DNA binding

2.5 × 10-2

3.6

11

7

1.6

10.1

Cytoskeletal organization

1.5 × 10-5

3.5

13

4

3.2

9.6

Actin filament-based process

2.5 × 10-8

6.0

1

11

-11.0

6.7

Microtubule cytoskeletong

3.3 × 10-2

3.0

9

5

1.8

7.9

Oxidoreductase activityf

5.1 × 10-2

1.8

3

2

1.5

2.8

Cell redox homeostasis

6.4 × 10-3

6.7

11

16

-1.5

15.2

Response-to-stress

8.9 × 10-2

1.4


Proteins were classified using the Gene Functional Annotation Tool of DAVID bioinformatics resources. a. Gene Ontology; b. Ratios are negative where the number of proteins down-regulated at 25°C (# DN) > the number of proteins up-regulated at 25°C (# UP) and positive where # UP > # DN; c. % = the number of proteins in this category out of 178 proteins; d. Modified Fisher Exact P-value (probability of obtaining this many differentially expressed proteins in the category by chance); e. Fold-enrichment; f. GO Molecular Function term. g. GO Cellular Component term.

Zieger et al. BMC Genomics 2011 12:630   doi:10.1186/1471-2164-12-630

Open Data