Table 1

Changes in protein expression following cold-adaptation as determined by LC/MS-based label-free protein quantitative analysis.

Protein priority

Peptide ID confidence

Multiple sequences

Number of proteins

Number significant changes

Maximum absolute fold change

Number increasing at 25°C

Number decreasing at 25°C


1

High

Yes

440

169 (15.5%)

1.70

73

96

2

High

No

364

50 (4.6%)

1.92

12

38

3

Moderate

Yes

7

4 (0.04%)

1.23

0

4

4

Moderate

No

278

81 (7.4%)

2.37

17

64

Overall

1089

304 (27.9%)

2.37

102 (9.4%)

202 (18.5%)


A total of 1089 proteins were identified by LC/MS and 27.9% of these had significantly increased or decreased in abundance following 25°C/72 h. Priorities 1-4 were assigned based on confidence in peptide identification (High and Moderate) and whether or not there are multiple amino acid sequences (Yes or No) identified. Please see references [24] and [26] for more details about how the protein priority is determined.

Zieger et al. BMC Genomics 2011 12:630   doi:10.1186/1471-2164-12-630

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