Hybrid assembly strategy pipeline. All 454 reads were assembled by using Newbler assembler with seed length 16, minimum overlap length 40 and minimum overlap identity 95%. All Illumina reads were assembled by using Velvet assembler with K-value 29. MIRA assembler was then used to generate the hybrid contigs with minimum overlap 40.
Jiang et al. BMC Genomics 2011 12:629 doi:10.1186/1471-2164-12-629