Table 7

Genes verified as DE from qPCR analyses

Ae. albopictus ID

Putative homolog

454 category

Fold-change

corrected p-value

Functional category


Aalb_oocyte_rep_c42113_trimmed

phosphoenolpyruvate carboxykinase (pepck)

DI

0.82

0.004

metabolism

Aalb_oocyte_rep_c2808_trimmed

Ecdysone inducible protein L2, putative (eip)

NDI

-0.85

0.005

endocrine signaling

Aalb_oocyte_rep_c41764_trimmed

Inhibitor of growth protein (ing1)

a priori

1.05

0.002

DNA replication/ transcription

Aalb_oocyte_rep_c38864_trimmed

Conserved hypothetical protein (AAEL004873)

NDI

0.48

0.004

unknown

Aalb_oocyte_rep_c36433_trimmed

Conserved hypothetical protein (AAEL008645)

DI

1.33

0.000

unknown

Aalb_oocyte_GH79BIP01BENVH_trimmed

Bhlhzip transcription factor bigmax

DI

0.87

0.010

DNA replication/ transcription

Aalb_oocyte_rep_c3449_trimmed

GPCR Methuselah Family

a priori

0.88

0.004

stress response

Aalb_oocyte_rep_c40438_trimmed

Receptor for activated C kinase, putative (rack1)

DI

0.86

0.003

endocrine signaling

Aalb_oocyte_rep_c18194_trimmed

Conserved hypothetical protein (AAEL012019)

DI

0.92

0.010

unknown

Aalb_oocyte_rep_c431_trimmed

phosphatidylethanolamine-binding protein (pebp)

a priori

0.88

0.000

morphogenesis


Information on each gene verified as differentially expressed is shown. Putative homologs were derived from BLAST searches (see Methods). "454 category" refers to the differential expression status assigned to the EST based on read counts: DI or NDI, significantly over-expressed under DI or NDI conditions, respectively; and a priori, not DE based on 454 read counts, but of a priori interest based on previous diapause research. Fold-change calculations are based on qPCR results, and were calculated as log2(DI-NDI), where DI and NDI are the average mRNA abundance for DI and NDI treatments, respectively (see Methods). The corrected p-value is derived from Student''s t-test of mRNA abundances derived from qPCR reactions.

Poelchau et al. BMC Genomics 2011 12:619   doi:10.1186/1471-2164-12-619

Open Data