Table 1

Gene expression fold-changes between M.bovis-infected (n = 8) and control (n = 8) based on microarray and real time qRT-PCR analyses

Gene symbol

Gene name

Gene description

Mean M.bovis-infected vs control group expression fold-change (microarray)

Mean M.bovis-infected vs control group expression fold-change (real time qRT-PCR)

Real time qRT-PCR

P-value


CASP1

Caspase 1 gene

A member of the cysteine-aspartic acid protease. Plays a role in the cell apoptosis

-1.45

-1.98

0.001


CD83

Cluster of differentiation 83 gene

A cell surface protein found in antigen presenting cells. Believed to play a role in antigen presentation or the cellular interactions that follow lymphocyte activation

+4.92

+5.88

< 0.001


CTLA4

Cytotoxic T-lymphocyte-associated protein 4 gene

A member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells

+3.20

+3.46

0.001


DEFB10

Beta-defensin 10 gene

Host defence response to bacterial infection; has antimicrobial activity

-3.38

-12.21

0.018


IFNB1

interferon, beta 1, fibroblast

Cytokine activity

Not DE

Not DE

> 0.05


IFNAR1

Interferon (alpha, beta and omega) receptor 1 gene

A type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta

Not DE

-2.20

< 0.001


IFNG

Interferon gamma gene

A soluble cytokine with antiviral, immunoregulatory and anti-tumor properties. A potent activator of macrophages

Not DE

Not DE

> 0.05


IFNGR1

Interferon gamma receptor 1 gene

Forms a heterodimer with interferon gamma receptor 2. Involved in binding of interferon gamma

Not DE

+2.23

< 0.001


IFNGR2

Interferon gamma receptor 2 gene

Forms a heterodimer with interferon gamma receptor 1. Involved in binding of interferon gamma

-1.32

Not DE

> 0.05


IL1A

Interleukin 1, alpha gene

A member of the interleukin 1 cytokine family produced by macrophages. Involved in various immune responses, inflammatory processes, and haematopoiesis

+1.89

+2.47

0.009


IL8

Interleukin 8 gene

A chemokine that mediates the inflammatory response

+2.15

+4.01

< 0.002


IL15

Interleukin 15 gene

A cytokine that regulates T and natural killer cell activation and proliferation

-1.49

-2.03

< 0.001


JAK1

Janus kinase 1 gene

A widely expressed membrane-associated phosphoprotein involved in the interferon-alpha/beta and -gamma signal transduction pathways

Not DE

Not DE

> 0.05


KIR3DS1

Killer cell immunoglobulin-like receptor, three domains, short cytoplasmic tail, 1

A transmembrane glycoprotein expressed by natural killer cells and some T cells. Involved in regulation of the immune response

-1.44

-2.63

0.005


MYD88

myeloid differentiation primary response gene (88)

A cytosolic adapter protein that functions as a signal transducer in the interleukin-1 and Toll-like receptor signalling pathways

-1.31

-2.55

< 0.001


PTPN2

protein tyrosine phosphatase, non-receptor type 2 gene

A member of the protein tyrosine phosphatase (PTP) family that is involved in a variety of cellular processes including cell growth, and differentiation

Not DE

-1.43

< 0.001


STAT1

signal transducer and activator of transcription 1, 91kDa gene

A transcriptional activator protein activated in response to cytokines and growth factors

+1.28

-1.27

< 0.001


STAT2

signal transducer and activator of transcription 2, 113kDa gene

A transcriptional activator protein activated in response to cytokines and growth factors

-1.23

-1.86

< 0.001


TLR3

Toll-like receptor 3 gene

An intracellular pathogen recognition receptor (PRR) that largely recognises viral pathogen-associated molecular patterns (PAMPs). Mediates the production of cytokines necessary for the development of effective immunity

-1.55

-1.63

0.001


TLR4

Toll-like receptor 4 gene

A cell-surface PRR that recognises PAMPs expressed by infectious agents. Mediates the production of cytokines necessary for the development of effective immunity

+2.41

Not DE

> 0.05


TREM1

Triggering receptor expressed on myeloid cells 1 gene

A receptor expressed on myeloid cells upon microbial infection. Amplifies neutrophil and monocyte-mediated inflammatory responses stimulating release of pro-inflammatory chemokines and cytokines

-1.70

-2.81

0.002


TYK2

Tyrosine kinase 2 gene

A member of the tyrosine kinase. A component of both interferon signalling pathways

Not represented on array

-1.60

< 0.001


TYROBP

TYRO protein tyrosine kinase binding protein gene

A transmembrane protein involved in cell signalling

-1.38

-1.98

< 0.001


Gene descriptions taken from GeneCards version 3 [82] and the NCBI Entrez Gene database [83]. For the microarray data, genes with fold-change differences in expression were significant after adjustment for multiple testing using the Benjamini-Hochberg method [31] (adjusted P ≤ 0.05). 'Not DE' indicates a gene was not differentially expressed between the two sample groups.

Killick et al. BMC Genomics 2011 12:611   doi:10.1186/1471-2164-12-611

Open Data