Assessing coverage of the O. fasciatus transcriptome. Randomly chosen subsets of increasing numbers of Titanium reads were used to generate progressively larger sub-assemblies. The number of reads in each sub-assembly (X axis) is plotted against the number of unique BLAST hits in each sub-assembly (left Y axis: black), and against the N50 isotig length (right Y axis: grey). For this analysis BLAST was performed against the SwissProt database. The number of unique BLAST hits plateaus when the assembly is composed of approximately 1.5 million reads. However, the N50 isotig length maintains an approximately constant rate of increase.
Ewen-Campen et al. BMC Genomics 2011 12:61 doi:10.1186/1471-2164-12-61