Additional file 9.

Validation of RNA-seq expression trends using a real time RT-PCR approach. Reciprocal (1/Ct) real time PCR values were averaged for each enzyme mentioned in Table 3. The multiplied by 10000 and log2 transformed. Similarly RNA-seq mean average values were log2 transformed and compared against real time RT-PCR results. A) A significantly strong correlation (R2 = 0.91686) of RNA-seq and real time PCR expression levels were observed for six enzymes. From left to right black boxes correspond to GGPPS, DXS, AACT, HMGR, MDD and IDS enzymes; B) Upon inclusion of the HDS expression data (blue box), the linear correlation of RNA-seq and real time PCR expression levels was still significant (R2 = 0.79392); C) Upon further addition of the CS expression data (red box) the linear correlation dropped significantly (R2 = 0.2173). Abbreviations of enzymes are as shown in Figure 6's legend.

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Barrero et al. BMC Genomics 2011 12:600   doi:10.1186/1471-2164-12-600