Additional file 1.

Assessment of reads using FastQC before trimming. A) Quality of reads per base. The central red line is the median base quality, the yellow box represents the inter-quartile range (25-75%), the upper and lower whiskers represent the 10% and 90% points and the blue line represents the mean base quality. B) Distribution of mean quality scores over all sequenced reads. C) The GC content distribution over all sequenced reads as compared against the theoretical GC distribution. The blip in GC content above the theoretical GC distribution is most likely due to the utilized primers at the 5' end of the reads used during RNA-seq library preparation and sequencing.

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Barrero et al. BMC Genomics 2011 12:600   doi:10.1186/1471-2164-12-600