Open Access Highly Accessed Methodology article

High resolution profiling of human exon methylation by liquid hybridization capture-based bisulfite sequencing

Junwen Wang, Hui Jiang, Guanyu Ji, Fei Gao, Mingzhi Wu, Jihua Sun, Huijuan Luo, Jinghua Wu, Renhua Wu and Xiuqing Zhang*

Author Affiliations

Beijing Genomics Institute at Shenzhen, Beishan Road, Shenzhen 518000, China

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BMC Genomics 2011, 12:597  doi:10.1186/1471-2164-12-597

Published: 8 December 2011

Additional files

Additional file 1:

Supplementary Tables 1-5. Table S1: Data statistics of YH and mDC from HLC-BS. Table S2: Assessment of the dropout of alleles of heterozygous genes from the YH LHC-BS data. Table S3: Statistics of the methylation statuses of 27 individual CpG sites that were analyzed by HLC-BS and BS-PCR. P values were calculated using the chi-square or Fisher's tests. Table S4: Analysis of the undetected target region characteristics. Table S5: BS-PCR primer information.

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Additional file 2:

Figure S1 LHC-BS read distribution along chromosome 12.

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Additional file 3:

Figure S2 Comparison of methylation rates between WGBS and LHC-BS. (a) The Pearson's correlation coefficient from the YH blood sample was 0.907, and the confidence interval was 0.902-0.912; (b) for the mDC cell line, the correlation coefficient was 0.925, and the confidence interval was 0.921-0.928. The y-axis shows the methylation rate of a cytosine as determined by the whole genome sequencing of bisulfite-treated DNA, and the x-axis shows the methylation level as determined by HLC-BS. This analysis was restricted to cytosines with at least nine reads in both samples.

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