CTCF can bind uniquely in different breast cell lines. CTCF binding was mapped in two ER-positive breast cancer cell lines (MCF-7 and ZR75-1) as well as an ER-negative breast cell line (MCF10A). A. Heatmap showing CTCF binding profiles in MCF-7, ZR75-1 and MCF10A cells. CTCF binding events that are shared between all three cell lines (I), that are present in only the ER-positive MCF-7 and ZR75-1 cells (II), as well as CTCF binding events are unique to MCF-7 (III) or ZR75-1 (IV) or MCF10A cells (V) are shown. The window represents -/+ 5 kb regions from the centre of the CTCF binding events. B. Examples of genomic regions that are: I, common CTCF binding events, II: CTCF binding events present in ER-positive cell lines only, III: MCF-7 unique, IV: ZR75-1 unique, and V: MCF10A-unique CTCF binding events.
Ross-Innes et al. BMC Genomics 2011 12:593 doi:10.1186/1471-2164-12-593