Open Access Highly Accessed Research article

Directional gene expression and antisense transcripts in sexual and asexual stages of Plasmodium falciparum

María J López-Barragán1, Jacob Lemieux1, Mariam Quiñones2, Kim C Williamson13, Alvaro Molina-Cruz1, Kairong Cui4, Carolina Barillas-Mury1, Keji Zhao4 and Xin-zhuan Su1*

Author Affiliations

1 Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 9000 Rockville Pike, Bethesda, Maryland 20892, USA

2 Bioinformatics and Computational Biosciences Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 9000 Rockville Pike, Bethesda, Maryland 20892, USA

3 Department of Biology, Loyola University Chicago, Chicago, Illinois 60660, USA

4 Systems Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, 9000 Rockville Pike, Bethesda, Maryland 20892, USA

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BMC Genomics 2011, 12:587  doi:10.1186/1471-2164-12-587

Published: 30 November 2011

Additional files

Additional file 1:

Novel junctions discovered after analysis of the bidirectional RNA-Seq libraries from seven time points of Plasmodium falciparum life cycle.

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Additional file 2:

Exons with high levels of antisense (-log P > 150) transcripts from the strand-specific libraries.

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Additional file 3:

Functional enrichment of genes with high levels of antisense transcripts.

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Additional file 4:

Splicing junctions detected using 1 or 10 bridging reads, respectively.

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Additional file 5:

New intro-exon junctions detected using reads from the strand-specific libraries and two previous studies.

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Additional file 6:

Genes expressed at levels significantly different from ring-stage (fold changes).

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Additional file 7:

Validation of splicing junctions and alternatively spliced events detected.

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