Table 1

Comparison of fold changes between microarray and qPCR

Accession No.

Gene Symbol

Microarray

qPCR


AB109635

HMGCR

-1.03

-0.81

AB196971

APCDD1

4.75

7.96

AF505881

SCX

-3.27

-1.94

AJ131110

TWIST2

4.61

6.77

BU279212

CBLN2

7.84

8.64

BX929635

NDP

-4.26

-1.64

BX931599

VIPR2

-2.90

-11.34

BX932694

MPZL2

5.51

4.16

BX933478

MXRA5

-2.69

-7.97

BX933888

C1QTNF3

-2.53

-2.28

BX935456

EGLN3

-3.04

-4.48

BX936211

TMEM116

4.26

5.94

CK611983

CSTA

4.49

7.92

CR385566

CLEC3B

-4.46

-3.58

D87992

ANPEP

-4.25

-6.29

M60853

THBS2

-5.15

-5.28

M64990

PTGS2

-6.78

-10.36

M80584

LUM

-3.14

-4.59

M87294

NPY

-3.73

-7.15

X87609

FST

-4.12

-10.04


The gene expression levels of 20 genes from microarray analysis were confirmed by qPCR. The expression levels were presented by fold changes values in microarray analysis, while, for qPCR, the values were calculated by 2-ΔΔCT method, which were comparable to fold changes in the microarray. All values are mean values determined by the calculations from three replicate assays.

Kong et al. BMC Genomics 2011 12:571   doi:10.1186/1471-2164-12-571

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