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Open Access Research article

Complex trait subtypes identification using transcriptome profiling reveals an interaction between two QTL affecting adiposity in chicken

Yuna Blum123, Guillaume Le Mignon124, David Causeur3, Olivier Filangi12, Colette Désert12, Olivier Demeure12, Pascale Le Roy12 and Sandrine Lagarrigue12*

Author Affiliations

1 INRA, UMR598, Génétique Animale, IFR140 GFAS, 35000 Rennes, France

2 Agrocampus Ouest, UMR598, Génétique Animale, IFR140 GFAS, 35000 Rennes, France

3 Agrocampus Ouest, Applied Mathematics Department, 35000 Rennes, France

4 ITAVI, F-75008, Paris, France

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BMC Genomics 2011, 12:567  doi:10.1186/1471-2164-12-567

Published: 21 November 2011

Abstract

Background

Integrative genomics approaches that combine genotyping and transcriptome profiling in segregating populations have been developed to dissect complex traits. The most common approach is to identify genes whose eQTL colocalize with QTL of interest, providing new functional hypothesis about the causative mutation. Another approach includes defining subtypes for a complex trait using transcriptome profiles and then performing QTL mapping using some of these subtypes. This approach can refine some QTL and reveal new ones.

In this paper we introduce Factor Analysis for Multiple Testing (FAMT) to define subtypes more accurately and reveal interaction between QTL affecting the same trait. The data used concern hepatic transcriptome profiles for 45 half sib male chicken of a sire known to be heterozygous for a QTL affecting abdominal fatness (AF) on chromosome 5 distal region around 168 cM.

Results

Using this methodology which accounts for hidden dependence structure among phenotypes, we identified 688 genes that are significantly correlated to the AF trait and we distinguished 5 subtypes for AF trait, which are not observed with gene lists obtained by classical approaches. After exclusion of one of the two lean bird subtypes, linkage analysis revealed a previously undetected QTL on chromosome 5 around 100 cM. Interestingly, the animals of this subtype presented the same q paternal haplotype at the 168 cM QTL. This result strongly suggests that the two QTL are in interaction. In other words, the "q configuration" at the 168 cM QTL could hide the QTL existence in the proximal region at 100 cM. We further show that the proximal QTL interacts with the previous one detected on the chromosome 5 distal region.

Conclusion

Our results demonstrate that stratifying genetic population by molecular phenotypes followed by QTL analysis on various subtypes can lead to identification of novel and interacting QTL.