Additional file 3.
Figure S3. Retention frequency based on aCGH data. (A) aCGH intensity data for human RH clone 12 along chromosome 2 matches historical PCR data well (red lines) but does show some loss. (B) Retention frequency of human donor genome across all 79 RH clones. Solid line is loess smoothed with parameter 0.02. (C) Retention frequency of chromosome 6 is relatively uniform except for the centromere (grey) which shows preferential retention. (D) The Tk1 gene (red arrow) is retained at 100% as expected for the selectable marker. (E) The difference in retention frequency between centromeric (grey bars) and noncentromeric (red bars) region for all chromosomes is statistically significant (Welch's t > 8.1, d.f. > 477, P < 10-15). (F) The X chromosome has ~50% retention frequency of the autosomes because the donor cell line was male. The Y chromosome has an apparently higher retention frequency than the X, probably because the Y has a proportionally higher percentage of centromeric sequence (cf. Figure S4E). Error bars s.e.m.
Format: PDF Size: 1MB Download file
This file can be viewed with: Adobe Acrobat Reader
Wang et al. BMC Genomics 2011 12:562 doi:10.1186/1471-2164-12-562