Open Access Research article

Pyrosequencing the transcriptome of the greenhouse whitefly, Trialeurodes vaporariorum reveals multiple transcripts encoding insecticide targets and detoxifying enzymes

Nikos Karatolos1*, Yannick Pauchet23, Paul Wilkinson2, Ritika Chauhan2, Ian Denholm1, Kevin Gorman1, David R Nelson4, Chris Bass1, Richard H ffrench-Constant2 and Martin S Williamson1

Author Affiliations

1 Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK

2 Biosciences, University of Exeter, Penryn, TR10 9EZ, UK

3 Max Planck Institute for Chemical Ecology, 07745 Jena, Germany

4 Molecular Science, University of Tennessee, Memphis, USA

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BMC Genomics 2011, 12:56  doi:10.1186/1471-2164-12-56

Published: 24 January 2011

Additional files

Additional file 1:

Characteristics of assembled Trialeurodes vaporariorum 454 contigs and BLASTx alignments against Drosophila melanogaster. (A,B) length and coverage of contigs, (C,D) percent identity and deduced amino acid alignment length for all blast hits to D. melanogaster predicted proteins (additional file 1.pdf)

Format: PDF Size: 76KB Download file

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Additional file 2:

Top BLAST hits in the NCBI nr database for each unique contig. Note that only the contigs that returned a BLAST result are shown in this file (additional file 2.xls)

Format: XLS Size: 5.4MB Download file

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Additional file 3:

E-value (A) and percentage similarity (B) distributions of the top BLAST hit for each contig of Trialeurodes vaporariorum (additional file 3.tiff)

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Additional file 4:

Names, corresponding contig numbers, amino-acid sequences and Acyrthosiphon pisum or Bemisia tabaci orthologues of contigs that encode detoxifying enzymes (additional file 4.xls)

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Additional file 5:

P450s nucleotide sequences (additional file 5.txt)

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Additional file 6:

CCEs nucleotide sequences (additional file 6.txt)

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Additional file 7:

GSTs nucleotide sequences (additional file 7.txt)

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Additional file 8:

Nucleotide sequences of target sites of the most important insecticide classes (additional file 8.txt)

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Additional file 9:

Single nucleotide polymorphisms (SNPs) arising due to nucleotide divergence between the two strains (TV1 and TV6) (additional file 9.xls)

Format: XLS Size: 31KB Download file

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