Proof-of-concept example: simulation of the E2F-RB network. The network structure and kinetic model are obtained from . A) Simulation of the kinetic model based on a fixed initial condition (provided in ) represents measurement at the single cell-resolution of the system. The response curves of serum stimulation on the different genes in the model are plotted. B) Assign a Gaussian distribution with small variances on the initial gene expression level of the untreated cells to represent the cell-cell variation in a clone. Simulation-results are computed by averaging the responses of 100 cells in a clone. C) Sampling of 100 clones under different, randomly generated initial conditions. The simulation results are shown as histograms of the expression level of the different genes, together with their ΔAIC values.
Wu et al. BMC Genomics 2011 12:547 doi:10.1186/1471-2164-12-547