A pooled screen for invasive capacity of miRNAs using the lentiviral library. (A) Schematic of the pooled invasion screen setup. In brief, after clonal infection (not shown here), cells with 40 individual miRNA constructs were pooled and either subjected to two rounds of an invasion assay or used as the control fraction. Of both fractions, integrated constructs were quantified by massively parallel sequencing to determine enrichment. (B) miRNAs were chosen for confirmation based on a combination (E × R > 1) of enrichment (E) after invasion and their abundance in the invasive fraction; expressed as the scaled number of reads (R). 23 out of 45 miRNAs were confirmed to enhance invasive capacity of HCT15 cells in final individual invasion assays.
Poell et al. BMC Genomics 2011 12:546 doi:10.1186/1471-2164-12-546