Lentiviral transduction of miRNAs in a high-content setup. Five different cell lines were infected with either empty vector miR-372, or let-7a. Cells were fixed 96 hours after infection and stained with Hoechst. Samples were analyzed with automated image analysis software. Four parameters were assessed: 1, number of nuclei per field; 2) nuclear size; 3) nuclear shape; 4) chromatin condensation. Graphs on the right show the quantification of the number of nuclei per field. Error bars represent standard deviations of triplicate wells. Four fields were counted per well.
Poell et al. BMC Genomics 2011 12:546 doi:10.1186/1471-2164-12-546