Overview of the miRNA library. (A) 660 annotated and 422 candidate miRNAs were cloned from their genomic loci into a lentiviral expression construct. From each construct, an individual batch of virus supernatant was created and portioned into individual tubes organized in a 96-well format. (B) Overlap between miRNAs included in the lentiviral library and miRNAs annotated in version 14 and version 17 of miRBase. The library contains constructs with variants or duplicates of some miRNA loci, which brings the total to 1120 constructs.
Poell et al. BMC Genomics 2011 12:546 doi:10.1186/1471-2164-12-546