Open Access Research article

Pyrosequencing of the Camptotheca acuminata transcriptome reveals putative genes involved in camptothecin biosynthesis and transport

Yongzhen Sun1, Hongmei Luo1, Ying Li1, Chao Sun1, Jingyuan Song1, Yunyun Niu1, Yingjie Zhu1, Liang Dong1, Aiping Lv2, Enzo Tramontano3 and Shilin Chen1*

Author Affiliations

1 The Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, P. R. China

2 Institute of Basic Theory, China Academy of Traditional Chinese Medicine, Beijing, China

3 Department of Applied Sciences in Biosystems, University of Cagliari, Monserrato (Cagliari), Italy

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BMC Genomics 2011, 12:533  doi:10.1186/1471-2164-12-533

Published: 30 October 2011

Additional files

Additional file 1:

Annotation statistics against public databases. Word document for the summary of the annotation result.

Format: DOC Size: 63KB Download file

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Additional file 2:

Gene Ontology analysis of the 454 sequencing library. TIFF document for the function categorization of the library against the Arabidopsis database.

Format: TIFF Size: 531KB Download file

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Additional file 3:

Gene discoveries for CPT biosynthesis against the Nr, Swissprot and Kegg databases. Excel document of specific information for mining genes in CPT biosynthesis.

Format: XLS Size: 50KB Download file

This file can be viewed with: Microsoft Excel Viewer

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Additional file 4:

Amino acid alignment between CaG10H and CrG10H. TIFF document of protein sequence alignment of CaG10H and CrG10H.

Format: TIFF Size: 1.3MB Download file

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Additional file 5:

Peptide alignment between CaSCS and CrSCS. TIFF document of protein sequence alignment of CaSCS and CrSCS.

Format: TIFF Size: 1.2MB Download file

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Additional file 6:

Transcripts of CYP450s discovered in this dataset. Excel document of all the discovered transcripts of cytochrome P450.

Format: XLS Size: 23KB Download file

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Additional file 7:

Classification of transcripts annotated to cytochrome P450s in this library. Word document of the classification of cytochrome P450s transcripts.

Format: DOC Size: 70KB Download file

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Additional file 8:

Amino acid alignment between the predicted CaPGD and RsSGD. TIFF document of the comparison of CaPGD and RsSGD.

Format: TIFF Size: 696KB Download file

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Additional file 9:

Primers used in this study. Excel document of all the designed primers in this study.

Format: XLS Size: 32KB Download file

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