Open Access Research article

Development of genomic resources for the narrow-leafed lupin (Lupinus angustifolius): construction of a bacterial artificial chromosome (BAC) library and BAC-end sequencing

Ling-Ling Gao12, James K Hane1, Lars G Kamphuis1, Rhonda Foley12, Bu-Jun Shi3, Craig A Atkins24 and Karam B Singh1245*

Author Affiliations

1 Plant Industry, Commonwealth Scientific and Industrial Research Organisation, Private Bag No. 5, Wembley WA 6913, Australia

2 Centre for Food and Genomic Medicine, Perth WA 6000, Australia

3 Australian Centre for Plant Functional Genomics, University of Adelaide, Glen Osmond SA 5064, Australia

4 School of Plant Biology, The University of Western Australia, Crawley WA 6009, Australia

5 The Institute of Agriculture, The University of Western Australia, Crawley WA 6009, Australia

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BMC Genomics 2011, 12:521  doi:10.1186/1471-2164-12-521

Published: 21 October 2011

Additional files

Additional file 1:

Sequences. Alignment of the truncated sequences of the eight β-conglutin genes of L. angustifolius.

Format: DOC Size: 354KB Download file

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Additional file 2:

Blast2GO. RAW Blast2GO gene ontology assignments to BAC-end sequences of L. angustifolius.

Format: XLS Size: 712KB Download file

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Additional file 3:

PlantGOSlim. Summary of GO term abundance in the 13985 trimmed L. angustifolius BAC-end sequences, filtered by the 'plant' GOSlim subset.

Format: XLS Size: 86KB Download file

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Additional file 4:

MIPS2GO. Details of GO term conversions into MIPS FunCAT IDS.

Format: XLS Size: 202KB Download file

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Additional file 5:

MIPS_FunCAT_full. Summary MIPS FUNCAT annotations: BES counts are cumulative but BESs are not counted twice. Counts of offspring terms contribute to the counts of parent terms. Percentages are given in terms of the total 13985 tested.

Format: XLS Size: 73KB Download file

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Additional file 6:

Summary of Gene Functions. Summary of the relative proportions of predicted gene functions in L. angustifolius, summarised using the first two ranks of the MIPS FunCAT classification system.

Format: DOC Size: 106KB Download file

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Additional file 7:

Summary of SSR motifs. Simple Sequence Repeats (SSRs) identified in L. angustifolius.

Format: XLS Size: 2MB Download file

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Additional file 8:

SSR primers. Simple Sequence Repeats (SSRs) identified in L. angustifolius. Class I > = 20 bp, Class II 12-19 bp; fwd: forward; rev: reverse.

Format: XLS Size: 776KB Download file

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Additional file 9:

Additional SSRs. Additional SSRs predicated using tandem repeats finder (Benson 1999) and less stringent minimum SSR identity (> 75%).

Format: XLS Size: 39KB Download file

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Additional file 10:

Markers used for the genetic mapping of NLL. Summary of 1104 markers used for the genetic mapping of NLL

Format: XLS Size: 169KB Download file

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Additional file 11:

Primer sequences tested. Primer sequences for the 24 tested Class I SSR markers with additional adapter sequence (underlined).

Format: XLS Size: 30KB Download file

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