Table 5

qRT-PCR to monitor gene expression after introducing a point mutation upstream of spr1021 (G-29T) and spr1887 (G-46T) in the S. pneumoniae 1974 and T-7 lines.

Strains/transformants

Mean 1974T/1974WT

spr1021 expression ratioa, b, c

Mean 1974T/1974WT

spr1887 expression ratioa, b, c


T-1974spr1021

3.6 (0.4)

1.2 (0.2)

T-7spr1021

3.4 (0.4)

1.2 (0.2)

T-7spr1021, spr1887

3.3 (0.5)

12.9 (0.2)

1974

1 (0.2)

1 (0.2)


aT (transformant); WT (wild-type)

bValues represent an average of three independent experiments.

cThe standard deviation is indicated in parentheses.

Billal et al. BMC Genomics 2011 12:512   doi:10.1186/1471-2164-12-512

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