Table 4

Functional analysis of mutations in genes spr0188, spr0196, spr0333, spr1021 and spr1887 in resistance to LNZ and other antibiotics in S. pneumoniae.

Strains/transformantsa

Locusb

No. of colonies tested

Mutation status 1974M1c,d

Mutation status Transformantsc,d

MIC LNZe

(μg/ml)

MIC CHLe

(μg/ml)

MIC FFCe

(μg/ml)


R6

0.5

3

2

T-R6spr0188

spr0188

8

A409G Y137H

A409G Y137H

0.5

3

3

T-R6spr0196

spr0196

8

T235G I79L

T235G I79L

0.5

3

2

T-R6spr1887

spr1887

8

G-46T

G-46T

0.5

3

2

1974

1

3

2

T-1974spr0333

spr0333

8

G626T G209V

G626T G209V

1

4

4

T-1974spr1021

spr1021

3

G-29T

G-29T

2

4

4

T-197423SrDNA1974M1 (T-7)

spr_rrnaDCBA23S

G2576T

G2576T

16

24

16

T-7spr0333

spr0333

4

G626T G209V

G626T G209V

16

24

16

T-7spr1021

spr1021

2

G-29T

G-29T

32

32

32

T-7spr1887

spr1887

4

G-46T

G-46T

16

24

16

T-7sp0188

spr0188

4

A409G Y137H

A409G Y137H

16

24

16

T-7sp0188, spr0196

spr0188

A409G Y137H

A409G Y137H

32

12

8

spr0196

T235G I79L

T235G I79L


a T stands for transformants; spr0188 (ribosomal protein L3); spr0196 (ribosomal protein L16); spr0333 (conserved hypothetical protein/rRNA methyltransferase) spr1021 (ABC protein-coding gene); spr1887 (patA ABC gene).

b The S. pneumoniae loci number (spr#) are according to the nomenclature of S. pneumoniae R6.

c In noncoding sequences, the number preceded by '-' indicates the position upstream of the ATG.

d When the mutations are within coding regions, the changes in amino acids is indicated in italics.

e LNZ (linezolid); CHL (chloramphenicol); FFC (florfenicol).

Billal et al. BMC Genomics 2011 12:512   doi:10.1186/1471-2164-12-512

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