Table 3

Relationship between the mutation status at domain V of every 23S rRNA gene and the level of resistance to linezolid and other antibiotics.

S. pneumoniae strains

23S rDNA status in PCR fragment/transformant

No. of colonies tested

spr_ rrnaD

spr_ rrnaC

spr_ rrnaB

spr_ rrnaA

MIC LNZ

(μg/ml)

MIC CHL

(μg/ml)

MIC FFC (μg/ml)

MIC PCG (μg/ml)

R6

4

W

W

W

W

0.5

3

1

0.023

T-R6^23SrDNAR6M2

G2576T/G2576T

3

M

W

W

W

1

6

2

0.023

T-R6^23SrDNAR6M2

G2576T/G2576T

4

M

W

W

M

2

12

2

0.023

T-R6^23SrDNAR6M2

G2576T/G2576T

4

M

M

W

M

4

16

8

0.023

T-R6^23SrDNAR6M2

G2576T/G2576T

4

M

M

M

M

8

24

16

0.023

1974

4

W

W

W

W

1

3

2

0.023

T-1974^{23SrDNA1974M1}

G2576T/G2576T

12

M

M

M

M

16

24

16

0.023

W (wild-type version); M (1974M1 version); LNZ (linezolid); CHL (chloramphenicol); FFC (florfenicol); PCG (penicillin G).

Billal et al. BMC Genomics 2011 12:512   doi:10.1186/1471-2164-12-512