Table 2

Common mutations found between the S. pneumoniae 1974M1 mutant and the transformants resistant to linezolid.

Name/function of genes

Locus Namea

1974M1b,c

T-1974T3b,c,d

T-R6T2b,e


23S rDNA

spr_rrnaD23S

G2576T

G2576T

G2576T

23S rDNA

spr_rrnaC23S

G2576T

G2576T

G2576T

23S rDNA

spr_rrnaB23S

G2576T

G2576T

G2576T

23S rDNA

spr_rrnaA23S

G2576T

G2576T

G2576T

50S ribosomal protein L3

spr0188

T409C Y137H

T409C Y137H

T409C Y137H

50S ribosomal protein L16

spr0196

A235G I79L

A235G I79L

A235G I79L

ABC transporter ATP-binding subunit

spr1021

G-29T

G-29T

Conserved hypothetical/rRNA methyltransferase

spr0333

G503T S168I

Sodium/hydrogen exchanger family protein

spr0573

G141A T47I

Conserved hypothetical protein

spr0855

G25A A9T

Fibronectin-binding protein-like protein A

spr0868

C617A S206I

Conserved hypothetical protein

spr1115

C29T T10I

Transcription antitermination factor

spr1820

G38T W13L

ABC transporter ATP-binding/membrane-spanning protein

spr1885

T490G S164R

ABC transporter ATP-binding/membrane-spanning protein

spr1887

G-32T


a The S. pneumoniae loci number (spr#) are according to the annotation of S. pneumoniae R6.

b When the mutations are within coding regions, the change in amino acids is also indicated in italics.

c In noncoding sequences, the number preceded by '-' indicates the position upstream of the ATG.

d DNA of mutant 1974M1 transformed into 1974 (T3 represents third level transformation).

e DNA of mutant 1974M1 transformed into R6 (T2 represents second level transformation).

Billal et al. BMC Genomics 2011 12:512   doi:10.1186/1471-2164-12-512

Open Data